AMENDMENT NO. 1 TO FOURTH AMENDED AND RESTATED INVESTOR RIGHTSAGREEMENT

Exhibit 4.7

AMENDMENT NO. 1 TO FOURTH AMENDED AND RESTATED

INVESTOR RIGHTS AGREEMENT

This Amendment No. 1 to Fourth Amended and Restated Investor Rights Agreement (this “Amendment”) to that certain Fourth Amended and Restated Investor Rights Agreement (the “IRA”) dated as of November 20, 2013, is entered into as of the 3^rd day of July, 2014 (the “Effective Date”), by and among Roka Bioscience, Inc., a Delaware corporation (the “Company”) and the investors listed on Exhibit A attached hereto (referred to hereinafter as the “Investors” and each individually as an “Investor”). All capitalized terms used herein and not otherwise defined shall have the meaning ascribed to such terms in the IRA.

RECITALS

WHEREAS, Section 5.2 of the IRA provides that, except as otherwise expressly provided in the IRA, the IRA may be amended or modified (or provisions of the IRA waived either generally or in a particular instance and either retrospectively or prospectively) only with the written consent of (a) the Company (with the approval of the Company’s Board of Directors) and (b) the holders of at least 51% of the votes attributable to the then outstanding shares of Series E Stock, Series D Stock, Series C Stock and Series B Stock, voting together as a single class on an as converted to Common Stock basis (the “Requisite Holders”); and

WHEREAS, the undersigned Investors are the holders of that number of shares of currently outstanding Series E Stock, Series D Stock, Series C Stock and Series B Stock as set forth on Exhibit A hereto, and therefore constitute the Requisite Holders; and

WHEREAS, the Company and the Investors desires to amend the IRA in accordance with Section 5.2 thereof as set forth herein.

NOW, THEREFORE, in consideration of the mutual covenants contained herein and other good and valuable consideration, the receipt and sufficiency of which are hereby acknowledged, the parties to this Amendment hereby agree as follows:

AMENDMENT

1. Amendment of IRA. Section 1.1(o) of the IRA is hereby deleted in its entirety and the following substituted in its place:

“(o) “Qualified IPO” means (i) a firm commitment underwritten public offering of shares of the Company’s Common Stock at a price per share to the public of at least $3.825 (adjusted for stock splits, stock dividends, recapitalizations and similar events, including any such events to be effected in connection with such offering) resulting in aggregate proceeds to the Company (net of the underwriting discounts or commissions and offering expenses) of not less than $50,000,000, or (ii) the closing of a firm commitment underwritten public offering of shares of Common Stock that is approved by the holders of at least fifty-one percent (51%) of the aggregate issued and outstanding shares of Series B Stock, Series C Stock, Series D Stock and Series E Stock, voting together as a single class on an as-converted to Common Stock basis.”

2. Reference to and Effect on the IRA.

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EXHIBIT A

LIST OF INVESTORS