Fasttrack Genetic Analysis Services Agreement No. MQ-20171213CG100, by and between Illumina, Inc. and the Registrant, dated December 13, 2017
[***] = Certain information contained in this document, marked by brackets, has been omitted because it is both not material and would be competitively harmful if publicly disclosed.
FASTTRACK GENETIC ANALYSIS SERVICES
5200 Illumina Way
San Diego, CA 92122, USA
Hereinafter referred to as Illumina
Hereinafter referred to as Personalis or Customer
December 13, 2017
June 30, 2019
FOR SMOOTH PROCESSING OF YOUR ORDER, ILLUMINA ASKS THAT YOU PLEASE REFERENCE THE ABOVE QUOTATION NUMBER ON ANY PURCHASE ORDER SUBMITTED AGAINST THIS QUOTATION.
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I. CUSTOMER INFORMATION
|Company or Institution Name:||Personalis|
|Address:||1330 Obrien Dr Menlo Park CA 94025-1436|
II. GENETIC ANALYSIS SERVICES PRICING INFORMATION
|Catalog#||Product Description||[***]||[***]||[***]||[***]||Subtotal (USD)|
|20023496||Infinium Global Screening Array - 24 V2.0 Kit Fast Track Service: BDCHP, CNSM, GSCA-24v2-0||[***]||[***]||[***]||[***]||[***]|
|Final Investment (Including shipping and insurance)||[***]|
Post Pricing Message:
Customer understands that the Infinium Global Screening Array - 24 V2.0 Kit Fast Track Service (20023496) is not yet available for ordering. Illumina will not accept orders against this Quotation and any purchase order placed against this Quotation for Infinium Global Screening Array - 24 V2.0 Kit Fast Track Service (20023496) will be non-binding on Illumina. Customer will be notified once the Infinium Global Screening Array - 24 V2.0 Kit Fast Track Service (20023496) is available for purchase and Illumina may issue a final quotation at that time based upon any updates to the product catalog number and delivery timeframes.
This Quotation is for informational purposes only and does not represent a commitment from Illumina on any product specifications or features, kits, or delivery date.
Customer agrees not disclose this pre-release quote or pricing to any third party until it is available for ordering.
[***] towards fulfillment of [***] and is only valid upon receipt of a purchase order for the full value of this Quotation and a fully executed subcontract and Pricing Agreement
Net 60 Payment terms shall apply to this Personalis subcontract with Illumina Fast Track Services
Pricing: Subject to the attached Terms and Conditions (Document 070-2003-010 31Jan08), upon delivery of the Results, Customer shall pay Illumina the amount listed above for each sample.
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III. CONDITIONS OF SALE
By this quotation Illumina offers to Customer the above-described Illumina Genetic Analysis Services. By submitting an order, Customer accepts the terms of this quotation, including Illuminas terms and conditions attached.
IV. HOW TO ORDER
To process this order, please email or fax the following:
1. This Quotation in its entirety
a. Please ensure that the Customer Information details are accurate on page 2
2. Your institutional Purchase Order referencing this quotation
(to the attention of):
Sales Contract Administration
Fax: +1 ###-###-####
Alt. Fax: +1 ###-###-####
Phone: +1 ###-###-####
Illumina will confirm receipt of the order within 24 hours
V. EXPIRATION OF OFFER
The offer contained in this document is revocable at the sole discretion of Illumina if not executed by Customer and a purchase order received by Illumina before 5:00 pm Pacific Time on the expiration date shown on page 1 of this quotation.
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FastTrack Genotyping Services
1. Samples. Customer agrees to supply Illumina with a complete and accurate sample manifest in accordance with Section 0 of this Service Description.
If Illumina determines in its sole discretion that any of the Samples do not meet the criteria set forth in this Service Description, Illumina will so inform Customer. At such time, Customer will be provided the option to (a) replace such Samples, or (b) proceed with such Samples as-is. In both (a) and (b), Customer shall pay Illumina full price for the Services to be performed with respect to such Samples, even if no successful Results are obtained for those Samples.
2. Results. Illumina shall deliver to Customer the Results as described below: SNP genotyping calls and scores for the assays performed on each Sample.
3. Delivery Dates. The delivery date(s) for the Results will be established at the start of the project, based on the number of Samples and current laboratory queue.
The following are minimum time frames for delivery of results:
For Infinium iSelect custom genotyping assays, within 90 days once a purchase order, loci selection and samples have all been received.
For standard genotyping studies using the Infinium assay, within 60 days of receipt of purchase order and samples, whichever occurs last. For studies larger than 1,000 samples, delivery date of the Results will be established at the start of the project, based on the current laboratory queue.
4. SNP Selection Process. For any custom assays that are to be developed by Illumina for supply of genotyping data, Customer agrees that, within 14 days of receipt of the purchase order, it will identify for Illumina a set of SNP loci (Loci) in accordance with the SNP Selection guidelines set forth in Section 5 of in this Service Description. Illumina and Customer must mutually agree upon the final Loci. Illumina will attempt to develop a functional SNP assay for each SNP locus provided by the Customer and agreed upon by Illumina. Upon the delivery of Loci by the Customer to Illumina, the Customer shall identify any of such Loci which are not publicly available.
5. Sample Requirements. Illuminas experience shows that Samples conforming to the following requirements are likely to yield excellent genotyping data.
To ensure that high quality data is produced, the Customer should submit a manifest of Sample characteristics. Illuminas laboratory uses standard operating procedures, and it is important to identify any variation in Sample preparation as early as possible. For each Sample, provide the gender, parental identification, and replicate sample identification. Please use the following format for the Sample Manifest.
DNA-plate barcode (e.g., GS0000777-DNA for GoldenGate, WG0000888-DNA for Infinium)
Well position of the Sample (e.g., A05)
Customer Sample ID (e.g., GS0000777-DNAA05_Name)
Species of the sample (e.g., Homo sapiens)
Gender of the individual (F: female, M: male, U: unknown) Comments
Volume in the well (e.g., 40 µL).
DNA concentration (50ng/µL or greater) measured by PicoGreen
Tissue source (e.g., cell line)
Extraction Method (e.g., Phenol/Chloroform)
WGA Method (if applicable, e.g., REPLI-g)
Mass of DNA used in WGA (if applicable, e.g., 50ng)
Mother (e.g., GS000777-DNAA06_Name1)
Father (e.g., GS000777-DNAA07_Name2)
Replicate(s) (e.g., GS0000777-DNAA08_Name3)
Before shipping your Samples to Illumina, please forward a file containing the Sample Manifest to your Illumina contact for review.
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To retain anonymity of the individuals from whom the Samples originated, Illumina provides barcoded microtiter plates and references the Samples by the plate number and well position. This allows a seamless interface with our robotic processes and the LIMS database. The Samples are placed into the supplied plates, sealed with a silicone cap, frozen, and shipped on dry ice. It is imperative that the DNA remain solidly frozen during shipping to avoid the possibility of cross contamination or degradation.
DNA Sample Criteria
DNA should be quantified using a double stranded, DNA specific method such as PicoGreen fluorescence.)
All DNAs must be at a concentration of 50 ng/µL or greater. The total amount of DNA required is dependent on the number of SNPs to be studied and is defined for each project. A table with typical DNA requirements is provided below.
DNA must be diluted in 10mM Tris/1mM EDTA.
A brief description of the DNA extraction protocol(s) should be included.
As a preliminary test, we ask for a few representative samples. We evaluate these test DNAs for performance quality on the Illumina genotyping platform. These samples are not part of the project and will be destroyed once evaluated.
|Assay||Project Type||Min. µg per |
|µL per sample|
|Infinium||Standard and iSelect||1.75||35|
Microtiter Plate Configuration
Illumina will provide barcoded, midi plates with corresponding lids.
Wells A01 and A12 must remain empty for Illumina DNA controls.
The lids must be sealed tightly and completely. We suggest the use of devices such as the MJ Research Roller for Microseal Film; catalog number MSR-0001 or the Corning Storage Mat Applicator; catalog number 3081.
Customer is responsible for maintaining a map of the DNA positions on each microtiter plate. The genotyping data will reference the barcode and well position of each DNA.
DNA must be solidly frozen prior to packing and shipment, and remain frozen. Plates must be shipped on sufficient dry ice to ensure that the samples remain frozen.
DNA should be shipped overnight express.
Illumina SNP Selection and Submission Requirements for Custom Genotyping Projects
lllumina SNP Format Guidelines
Queries for SNP selection can be submitted to obtain designability information. In addition, for human studies, data from the currently supported dbSNP version will be returned. The following input file types are supported:
SNP list. Customers can submit SNPs for human studies using the RS identifiers.
Gene list. Gene lists (and distance upstream and downstream from the designated gene) can be used as an input to obtain a list of SNPs within the gene region for human studies.
Region list. A list of markers that act as start and stop region identifiers can be used to query SNPs for human studies. As with the gene list, the distance upstream and downstream (in base pairs) can also be designated in the input format.
Sequence List. For human studies and a supported list of species (e.g., mouse, dog, chicken, ), a list of sequences, chromosomal designations and coordinates can be input. Assays for non- supported species can be designed as well using this submission format. Sequence lists require the use of IUPAC code for any neighboring polymorphisms with the targeted SNP in brackets (e.g., [A/G]) with a minimum of 50bp of sequence on either side of the targeted SNP.
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SNP Selection Additional Support
In addition to data on designability and minor allele frequency provided through Illuminas Assay Design Tool (ADT), Illuminas Fast-Track Genotyping Services is able to provide the following additional support in SNP selection:
Tag Selection. We use genotype data from Phase I+II of the International HapMap Project to complete a tag selection. User inputs include overall regions to target, SNPs per bin (based on r^2 threshold), minor allele frequency minimum, and population of interest (CEU, CHB, JPT, YRI).
Spacing Optimization. Illuminas automated spacing selection tool can help target the SNPs within a list that give optimal spacing balanced with minor allele frequency (and, if desired, mandatory SNPs).
Annotation. Illumina can provide annotation (contig, refseq genes, and distance to the closet intron/exon boundary on any RS list in human studies.
SNP Analysis and Selection Process
As part of the SNP selection process, SNP sequences are analyzed for assay fitness. Examples of some criteria considered for assay design ability include:
The SNP sequences are not from duplicated sequences within the genome.
The SNP sequence is not in a repeated element.
The AT and GC content is acceptable.
Tm is acceptable.
A note regarding iSelect Custom Infinium SNP selection
All SNP classes use the Infinium chemistry. The following SNPs require a minimum of one bead type:
A/C, A/G, T/C, and T/G. SNPs requiring two bead types include A/T and C/G SNPs
After analysis Customer will receive a file of the markers and their design score for the Illumina assay along with minor allele frequency and any additional supported information requested (e.g., annotation or tag selection) when available. The Customer will be given the opportunity to review, approve and edit the selected SNPs. After Customer approval of the SNPs, oligo manufacturing will begin. The number of SNPs present in the final data set will depend on the assay conversion rate. It is expected that some assays will fail, and therefore no data will be delivered for these SNPs.
For Custom Infinium projects, the minimum manufacturing locus conversion rate is 80%. The assay success rate depends on the markers that are selected during the design phase. If a majority of chosen markers have previously been validated on the Infinium platform (design score of 1.1) the final locus conversion rate may be very high. Selected markers with low design scores will however reduce the locus conversion rate.
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ILLUMNA TERMS AND CONDITIONS SERVICES
(NON-CLINICAL LABORATORY SERVICES)
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