Lease Amendment Agreement No. 2, dated April 1, 2023, by and between enGene and NeoMed Institute

Exhibit 10.19

LEASE AMENDMENT AGREEMENT NO 2 dated effective April 1, 2023

(the “Amendment”)

WHEREAS the Lessor and the Lessee entered into the certain Lease Agreement dated November 1^st, 2021 (the “Lease”);

WHEREAS the Lessor and the Lessee modified the Lease with an Amendment dated November 1^st, 2022 (the “Amendment #1”);

WHEREAS the Parties wish to modify the Lease with this Amendment (the “Amendment #2”), addressing removal of room #1310.

NOW, THEREFORE in consideration of the foregoing and the mutual covenants herein contained, and for other good and valuable consideration, the receipt and sufficiency of which are hereby acknowledged, the Parties hereby agree as follow:

The Lease is hereby amended as follows:

1. Leased Premises

Section 1 of Schedule A of the Lease under Leased Premises is hereby deleted and replaced by the following:

2. Plan of the leased premises

Appendix 2.1 (Plan of the leased premises) is hereby repealed and replaced as follows:

Basement

Ground Floor

Second Floor

Other than as expressly provided above, the Lease remains unaffected by this Amendment and continues in full force and effect.

Governing law and jurisdiction for this Amendment are in accordance with Section 9.2 of the Lease.

The parties have requested that this Amendment be exclusively prepared in English. Les parties ont requis que cette entente ainsi soit exclusivement rédigée en anglais.

This Amendment may be executed electronically, and/or in counterparts and such counterparts will for all purposes constitute one agreement binding the parties. The Lessor and the Lessee acknowledge and agree that this Amendment may be communicated by email with PDF attachments or signature pages, which will be equally binding and duly accepted as an original agreement.

[Signature page follows.]

IN WITNESS WHEREOF THE PARTIES HAVE SIGNED THIS AMENDMENT EFFECTIVE AS OF THE EFFECTIVE DATE ABOVE AND BINDING ON THE PARTIES.