EX-101 INSTANCE DOCUMENT

Exhibit 10.25

SECOND AMENDMENT TO SECOND LIEN CREDIT AGREEMENT

          THIS SECOND AMENDMENT, dated as of December 7, 2010 (this “Amendment”), to the Credit Agreement referred to below, by and among ALERE US HOLDINGS, LLC (f/k/a IM US Holdings, LLC), a Delaware limited liability company (the “Borrower”), the other Loan Parties signatory hereto (the “Loan Parties”), ALERE INC. (f/k/a Inverness Medical Innovations, Inc.), a Delaware corporation (“Holdings”), the Lenders signatory hereto (collectively, the “Lenders”) and GENERAL ELECTRIC CAPITAL CORPORATION, as collateral agent and administrative agent for the Lenders (in such capacity, the “Administrative Agent”).

W I T N E S S E T H

          WHEREAS, the Borrower, Holdings, the Administrative Agent, the Lenders party thereto from time to time are parties to that certain Second Lien Credit Agreement, dated as of June 26, 2007 (as amended, restated, supplemented or otherwise modified from time to time, the “Credit Agreement”); and

          WHEREAS, the Borrower has requested, and the Administrative Agent and Required Lenders have agreed, to provide for certain amendments to the Credit Agreement, on the terms and subject to the conditions set forth herein.

          NOW THEREFORE, in consideration of the promises and for other good and valuable consideration, the receipt, adequacy and sufficiency of which are hereby acknowledged, the parties hereto agree as follows:

          1. Definitions. Capitalized terms not otherwise defined herein, including in the recitals, shall have the meanings ascribed to them in the Credit Agreement, as amended hereby.

          2. Amendments to Credit Agreement. The Credit Agreement is hereby amended as of the Second Amendment Effective Date (as hereinafter defined) as follows:

          (a) Article I of the Credit Agreement is hereby amended by deleting the word “and” immediately after clause (b)(viii) of the definition “Excess Cash Flow” therein and inserting a new clause (b)(x) immediately after clause (b)(ix) of such definition as follows:

“, and (x) the aggregate consideration paid in cash by the Group Members for all Permitted Stock Repurchases during such period but excluding the portion thereof financed with long-term Indebtedness (other than the Obligations) or with proceeds of the issuance of common stock of Holdings,”

          (b) Article I of the Credit Agreement is hereby amended by adding the following new definition in the appropriate alphabetical order as follows:

“‘Permitted Stock Repurchases’ means any repurchase, redemption, retirement or other purchase by Holdings of Stock or Stock Equivalents of Holdings satisfying each of the following conditions: (a) as of the date of consummation of any such repurchase, redemption, retirement or other purchase and after giving effect thereto on such date, no Default or Event of Default shall be continuing, (b) both immediately before and immediately after giving effect to such repurchase, redemption, retirement or other purchase, the Loan Parties and their

Subsidiaries shall have First Lien Revolver Availability (as defined below) and available cash and Cash Equivalents of at least $150,000,000 in the aggregate, and (c) the aggregate consideration paid by the Group Members for all such repurchases, redemptions, retirements or other purchases after the Second Amendment Effective Date shall not exceed $200,000,000. For purposes of this definition, “First Lien Revolver Availability” shall mean the maximum amount of unused First Lien Revolving Credit Commitments that would be available for borrowing of First Lien Revolving Loans if after giving effect to such First Lien Revolving Loans, Borrower would be in compliance with the Consolidated Leverage Ratio covenant set forth in Section 5.1 of the First Lien Credit Agreement on a Pro Forma Basis as of the last day of the last Fiscal Quarter for which Financial Statements have been delivered hereunder for the four Fiscal Quarter period ending on such day (as if the Borrower had incurred such First Lien Revolving Loans on the first day of such period).

‘Second Amendment Effective Date’ means December 7, 2010.”

          (c) Section 6.1 of the Credit Agreement is hereby amended by inserting a new Section 6.1(l) immediately following Section 6.1(k) therein as follows:

               “(l) Permitted Stock Repurchases. Together with each delivery of any Financial Statements pursuant to clause (a) above, a summary of all Permitted Stock Repurchases made during such Fiscal Quarter, including the applicable repurchase dates of, and consideration paid by the Group Members for, such Permitted Stock Repurchases, and a calculation of the aggregate consideration paid by the Group Members for all Permitted Stock Repurchases made since the Second Amendment Effective Date through the end of such Fiscal Quarter.”

          (d) Section 8.5 of the Credit Agreement is hereby amended by (i) replacing each reference to “clause (c)” in the lead in paragraph thereof with the words “clause (c) or clause (e)”, and (ii) replacing the period at the end of Section 8.5(d) with “; and” and inserting a new Section 8.5(e) immediately following Section 8.5(d) therein as follows:

               “(e) Permitted Stock Repurchases, and cash dividends or other distributions on the Stock of Subsidiaries of Holdings to Holdings paid, declared and used solely for such purposes.”

          (e) Section 8.8(b)(ii) of the Credit Agreement is hereby amended and restated as follows:

               “(ii) issuing and selling its own Stock or Stock Equivalents, and repurchasing, redeeming, retiring or otherwise purchasing its own Stock or Stock Equivalents,”

          3. Remedies. This Amendment shall constitute a Loan Document. The breach by any Loan Party of any representation, warranty, covenant or agreement in this Amendment shall constitute an immediate Event of Default hereunder and under the other Loan Documents.

          4. Representations and Warranties. To induce the Administrative Agent and the Required Lenders to enter into this Amendment, each of Holdings, the Borrower and the other Loan

Parties represents and warrants to the Administrative Agent and the Lenders on and as of the Second Amendment Effective Date that:

               (a) The execution, delivery and performance by the Borrower and Holdings of this Amendment and the performance of the Credit Agreement, as amended by this Amendment (the “Amended Credit Agreement”), and the acknowledgment of this Amendment by the other Loan Parties signatory hereto: (i) are within such Loan Party’s corporate or similar powers and, at the time of execution thereof, have been duly authorized by all necessary corporate and similar action (including, if applicable, consent of the holders of its Securities), (ii) do not (A) contravene such Loan Party’s Constituent Documents, (B) violate any Requirement of Law, (C) conflict with, contravene, constitute a default or breach under, any material Contractual Obligation of any Loan Party or any of their respective Subsidiaries, other than those which could not reasonably be expected to have either individually or in the aggregate, a Material Adverse Effect, or (D) result in the imposition of any Lien (other than a Permitted Lien) upon any property of any Loan Party or any of their respective Subsidiaries and (iii) do not require any Loan Party or any of their respective Subsidiaries to obtain any Permit of, or make any filing with, any Governmental Authority or obtain any consent of, or notice to, any Person, prior to the Second Amendment Effective Date or, if not obtained, made or complied with, such failure could not, reasonably be expected to have either individually or in the aggregate, a Material Adverse Effect.

               (b) This Amendment has been duly executed and delivered by or on behalf of the Borrower and Holdings and acknowledged by each other Loan Party.

               (c) Each of this Amendment and the Amended Credit Agreement is the legal, valid and binding obligation of the Borrower and Holdings and is enforceable against the Borrower and Holdings in accordance with its terms, except as may be limited by applicable bankruptcy, insolvency, reorganization, moratorium, fraudulent transfer or other similar laws affecting creditors’ rights generally or by general equitable principles relating to enforceability.

               (d) No Default or Event of Default has occurred and is continuing or would result after giving effect to the provisions of this Amendment.

               (e) No action, claim or proceeding is now pending or, to the knowledge of any Loan Party, threatened against such Loan Party, at law, in equity or otherwise, before any court, board, commission, agency or instrumentality of any foreign, federal, state, or local government or of any agency or subdivision thereof, or before any arbitrator or panel of arbitrators, which (i) challenges any Loan Party’s right or power to enter into or perform any of its obligations under this Amendment, the Amended Credit Agreement, or any other Loan Document to which it is or will be, a party, or the validity or enforceability of this Amendment, the Amended Credit Agreement or any other Loan Document or any action taken thereunder, or (ii) has a reasonable risk of being determined adversely to such Loan Party and that, if so determined, could reasonably be expected to have a Material Adverse Effect after giving effect to this Amendment.

               (f) The representations and warranties of the Loan Parties set forth in any Loan Document are true and correct in all material respects (provided that if any representation or warranty is by its terms qualified by concepts of materiality, such representation is true and correct in all respects) on and as of the date hereof or, to the extent such representations and warranties expressly relate to an earlier date, on and as of such earlier date.

          5. No Waivers/Consents/Amendments. Except as expressly provided herein: (a) the Credit Agreement and the other Loan Documents shall be unmodified and shall continue to be in full force and effect in accordance with their terms and (b) this Amendment shall not be deemed a waiver of

any term or condition of any Loan Document and shall not be deemed to prejudice any right or rights which the Administrative Agent or any Lender may now have or may have in the future under or in connection with any Loan Document or any of the instruments or agreements referred to therein, as the same may be amended, restated, supplemented and otherwise modified from time to time.

          6. Affirmation of Obligations. Each of the Loan Parties hereby acknowledges, agrees and affirms: (a) its obligations under the Credit Agreement and the other Loan Documents, including, without limitation, its guaranty obligations thereunder, (b) that such guaranty shall apply to the Obligations in accordance with the terms thereof, (c) the grant of the security interest in all of its assets pursuant to the Loan Documents and (d) that such Liens and security interests created and granted are valid and continuing and secure the Obligations in accordance with the terms thereof.

          7. Outstanding Indebtedness; Waiver of Claims. Each of the Loan Parties hereby acknowledges and agrees that as of November 19, 2010, the principal amount outstanding on the Term Loan is $250,000,000. Each of the Borrower and the other Loan Parties hereby waives, releases, remises and forever discharges the Administrative Agent, any Lender and each other Indemnitee from any and all claims, suits, actions, investigations, proceedings or demands arising out of or in connection with the Credit Agreement or any other Loan Document (collectively, “Claims”), whether based in contract, tort, implied or express warranty, strict liability, criminal or civil statute or common law of any kind or character, known or unknown, which the Borrower or any other Loan Party ever had, now has or might hereafter have against the Administrative Agent, any Lender, or any other Indemnitee which relates, directly or indirectly, to any acts or omissions of the Administrative Agent, any Lender, or any other Indemnitee on or prior to the Second Amendment Effective Date; provided, that neither the Borrower nor any other Loan Party waives any Claim solely to the extent such Claim relates to the Administrative Agent’s or any Lender’s gross negligence or willful misconduct.

          8. Fees and Expenses. The Borrower and Holdings agree to pay to the Administrative Agent on the Second Amendment Effective Date for the benefit of each Lender executing this Amendment, a consent fee equal to 25 basis points of the aggregate outstanding principal amount of such Lender’s Term Loan (the “Consent Fee”). The Borrower hereby reconfirms its obligations pursuant to Section 11.3 of the Credit Agreement to pay and reimburse the Administrative Agent for all reasonable costs and expenses (including, without limitation, reasonable fees of counsel) incurred in connection with the negotiation, preparation, execution and delivery of this Amendment and all other documents and instruments delivered in connection herewith.

          9. Amendment Effectiveness. Upon satisfaction in full in the judgment of the Administrative Agent of each of the following conditions, this Amendment shall be deemed effective as of the Second Amendment Effective Date:

               (a) Amendment. The Administrative Agent shall have received copies of signature pages to this Amendment, duly executed and delivered by the Administrative Agent, the Borrower, Holdings and the Required Lenders and acknowledged by each of the other Loan Parties, with originals to follow promptly thereafter.

               (b) Representations and Warranties; No Defaults. The following statements shall be true on such date: (i) the representations and warranties set forth in any Loan Document shall be true and correct in all material aspects (provided that if any representation is by its terms qualified by concepts of materiality, such representation shall be true and correct in all respects) on and as of the Second Amendment Effective Date, to the extent such representations and warranties expressly relate to an earlier date, on and as of such earlier date and (ii) no Default or Event of Default shall be continuing.

               (c) Payment of Costs and Expenses. The Borrower shall have paid to the Administrative Agent, for the account of the Administrative Agent, its Related Persons or any Lender, all fees and reimbursement of costs or expenses, in each case, due and payable under the Loan Documents on or before the Second Amendment Effective Date (including, without limitation, reasonable legal fees and expenses of the Administrative Agent and the Consent Fee).

               (d) Third Amendment to First Lien Credit Agreement. The Administrative Agent shall have received two (2) copies of the Third Amendment to First Lien Credit Agreement, dated as of the date hereof, duly executed and delivered by the parties signatory thereto and effective in accordance with the terms thereof.

          10. Governing Law. This Amendment and the rights and obligations of the parties hereto, shall be governed by, and construed and interpreted in accordance with, the law of the State of New York.

          11. Counterparts. This Amendment may be executed by the parties hereto on any number of separate counterparts and all of said counterparts taken together shall be deemed to constitute one and the same instrument.

[SIGNATURE PAGES FOLLOW]

     IN WITNESS WHEREOF, this Amendment has been duly executed as of the date first written above.

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          Each of the undersigned Loan Parties hereby (i) acknowledges this Amendment and (ii) confirms and agrees that its Obligations under the Loan Documents shall continue without any diminution thereof and shall remain in full force and effect on and after the effectiveness of this Amendment:

ACKNOWLEDGED, CONSENTED and AGREED to as of the date first written above:

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